Ensure your greenhouse is suitable for truffle cultivation. Your greenhouse must get at least six hours of sunlight every day and have excellent drainage; the truffle-inoculated oak trees cannot be allowed to sit in standing water. Make sure the site's soil has a pH level between 6.5 to 8.0. If the pH is below 6.5, add lime to acidify the soil; if it is above 8.0, add sulfur to decrease the soil's acidity. Aerate the soil; make sure it is not packed or sandy.
Plant your truffle-inoculated oak trees at least 2 feet deep and in holes that are about twice as big as the root balls. Plant them about 4 feet apart to ensure enough growing room when the trees mature.
Water the trees enough to soak the ground around them at least 2 inches deep every three days.
Check the pH level of the soil frequently to make sure it is between 6.5 and 8.0.
Mulch the trees with small amounts of mulch -- just enough to cover the root area -- three or four times per year. Don't build a "volcano" cone of mulch, as this can cause the truffles and tree to rot from excess moisture.