Sterilize your pruning tools. Dip them either in rubbing alcohol, or a solution of 10 percent bleach and 90 percent water. Shake off the excess liquid and allow them to air dry. If trimming multiple plants, sterilize the tools after pruning each plant.
Remove the suckers from your muscadine plants. Suckers are small shoots that grow near the bases of plants – these shoots suck up nutrients before they can get to the main body of the plant. Cut them as close to the trunk as possible with a pair of clean garden shears.
Prune all side shoots with a pair of pruning shears during the dormant season, beginning around the middle of January. Count the buds from the end of each shoot – make your cut at about the third bud on each shoot. This will promote new growth and fruit production the following season.
Check the main plant for dead wood. It is perfectly normal for a healthy plant to have branches that have died while the rest of the plant goes on living. Look for brittle branches and nick them with your fingernail. If the wood is brown, cut away the branch with a pair of loppers. Use a pruning saw for particularly thick branches.